Latest article: |
Natural Products Targeting Oncology Pathways
04 May 2010: In this application note, BioFocus present three examples for natural compounds targeting specific tumor pathways: geldanamycin, borrelidin and fredericamycin. Gram quantities of the compounds are generated using microbial producers and semi-synthesis subsequently generates focused libraries of natural compound analogs. In vivo and in vitro protocols are then used to test potential anticancer properties of the compounds.
BioFocus
|
|
|
Recent articles: |
|
A Label-free Cell-based Functional Assay for Identification and Characterization of TRPV1 Channel Modulators
29 Mar 2010: Modulation of TRPV1, a nonselective cation channel, provides a potentially novel approach to treatment of a variety of disorders, such as chronic pain and inflammation. This poster presents a label-free BIND® cell-based assay for identification and characterization of TRPV1 modulators, allowing detection of the weak response induced by the putative natural activator anandamide and NADA.
SRU Biosystems
|
Rapid Production of Cells for Screening Voltage-Gated Ion Channels in Automated Electrophysiology Assays Using the MaxCyte® STX™ Scalable Transfection System
29 Mar 2010: This application note by MaxCyte presents examples of ion channel screening using CHO K1 cells that were transiently transfected with plasmids encoding voltage gated potassium channels. The MaxCyte® STX™ Scalable Transfection System enables rapid production of cells for high throughput ion channel assays and cells are shown to exhibit good membrane integrity.
MaxCyte
|
Live Cell Imaging of Drug Effects on Golgi Morpholgy Using the CELLview™ Cell Culture Dish
01 Mar 2010: This application note by Greiner Bio-One shows that performing live-cell imaging experiments in parallel in CELLview™ dishes with four compartments allows a direct comparison of the speed and timing of drug effects on the Golgi apparatus. GalNAc-GFP cells can be easily imaged at multiple positions without cell death or focus problems.
Greiner Bio-One GmbH
|
Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin
22 Feb 2010: SPR has been demonstrated to be a powerful optical technique for bioaffinity studies at the solid/solution interface. This application note describes the kinetic studies of ferulic acid and bovine serum albumin (BSA) pre-immobilized onto an alkanethiol self-assembled monolayer (SAM)-modified gold sensor chips.
Biosensing Instrument
|
Establishing a Cell Culture Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) for Screening G-Protein Coupled Receptors
09 Feb 2010: Time-resolved fluorescence resonance energy transfer (TR-FRET) has become a popular technique in the field of high-throughput screening. This application note demonstrates the TR-FRET kit from Cisbio, the IP-One HTRF assay, under standard high-throughput screening conditions. The IP-One HTRF assay was shown to be a stable cell culture time-resolved fluorescence assay for screening G-Protein coupled receptors.
Greiner Bio-One GmbH
|
Tag-lite®: New HTRF® Cellular Platform for Cell Surface Receptors’ Study and Screening
09 Feb 2010: In a recent publication, Maurel et al presented the combination of Cisbio’s HTRF and SNAP-and CLIP- Tag technologies to investigate cell-surface protein-protein interaction. In this study, an application for GPCR oligomerization was accurately documented and the advantages of using the Cisbio HTRF-based cell-surface receptor platform presented. Applications such as highly selective and non-radioactive ligand binding assays are described and the 7TM dimerization model can properly be inv
Cisbio Bioassays
|
Novel Flash Instrument Improves Sample Purity
27 Jan 2010: This application note demonstrates the Reveleris™ system has the ability to detect non-chromophoric compounds, synchronize three detection signals for smart fraction collection and more accurately represent mass balance and reaction yields.
Grace Davison Discovery Sciences
|
Novel Dual Detection Flash Instrument Improves Sample Purity and Productivity
26 Jan 2010: This application note demonstrates superior resolving power of the Reveleris™ system. Detecting non-UV active compounds improves sample purity and recovery while eliminating unnecessary post-purification steps.
Grace Davison Discovery Sciences
|
Application of SPR to Bacteriology: Endotoxin/Protein Interaction Studies
22 Jan 2010: This application note describes a novel application of SPR to the detection of endotoxin via its interaction with the CD-14 protein. This shows that SPR is a suitable technique for monitoring binding of endotoxins to proteins, antibiotics, and other species.
Biosensing Instrument
|
adme-toxicology
